genetic diversity of aegilops triuncialis l. accessions of iran revealed by microsatellite markers

نویسندگان

علی اشرف مهرابی

دانشیار، گروه زراعت و اصلاح نباتات، دانشکدۀ کشاورزی، دانشگاه ایلام سمیرا محمدی

کارشناس ارشد گروه زراعت و اصلاح نباتات، دانشکدۀ کشاورزی، دانشگاه ایلام صغری ولیان

کارشناس ارشد بیوتکنولوژی، دانشکدۀ کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات (تهران) محمود خسروشاهلی

استاد بیوتکنولوژی، دانشکدۀ کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات (تهران)

چکیده

in order to study the genetic diversity among 35 accessions of ae. triuncialis l. from17 ssr loci of 56 primer pairs originated from a and d genomes of bread wheat were used. seventy one fragments amplified totally and 68 of them were polymorph between genotypes. allele number ranged from 1 to 8 with average 4.18 per locus. pic value of markers was from 0.593 (xgwm-30-2d, xgwm-383-3d, xgwm-654-5d) to 0.861(xgwm-156-5a) and marker index varied from 1.779 to 6.888. average genetic distance of genotypes was 0.859. two accessions from lorestan and khoozestan with least genetic distance (0.231) was the most similar genotypes. cluster analysis was not able to separate accessions from each other.  no relationships between genetic and geographic variation indicates high amount of genetic diversity among genotypes. all molecular variation was from within groups which indicate no specific ssr alleles for accessions. this genetic diversity and transferability of known ssrs in cultivated could be assigned as useful tools to management of wild wheat genetic resources.

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عنوان ژورنال:
علوم گیاهان زراعی ایران

جلد ۴۶، شماره ۲، صفحات ۲۳۷-۲۴۵

کلمات کلیدی
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